diagnosticsgenome.com Home Products Molecular Regents PCR PreMix 2X Hot Start PCR-PreMix2 x PCR Hot Start PRE-MIX is an optimized, ready to use PCR mixture of Taq DNA Polymerase, antibodies to Taq DNA Polymerase, PCR buffer, MgCl2 and dNTP's. 2x PCR pre-mix contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates and is also very suitable for Real Time PCR. Tested for absence of endodeoxyribonucleases and exodeoxyribonucleases and is tested in amplification of a single gene copy.
SPECIFICATIONS : Supplied as 2x,in liquid form so as to ensure that you can add your primers & Template DNA volumes to desired levels.
APPLICATIONS : Suitable for Complex genomic cDNA templates, low copy number targets, large number of thermal Cycles, multiplex PCR. Quantitation of viral loads by Real Time PCR etc.
STORAGE : Recommended storage is at-20°C.
SUGGESTED DILUTION PROTOCOL FOR PCR :
| Component | 50 µl reaction Volume | 25 µl reaction volume | ||
| Volume | Final Conc. | Volume | Final Conc. | |
| 2 x PCR Premix | 25 µl | 1 X | 12.5 µl | 1 X |
| Forward primer | Variable | 0.1-1 µM | Variable | 0.1-1 µM |
| Reverse Primer | Variable | 0.1-1 µM | Variable | 0.1-1 µM |
| Template DNA | Variable | 10pg-1 µg | Variable | 10pg-1 µg |
| Sterile Deionized water | Up to 50 µl | ---- | Up to25 µl | ---- |
| Suggested Cycling protocol : | |||
| Initial Denaturation: | 95°C | 5 minutes | |
| Cycling | 95°C | 20 secs | |
| Annealing | 30 secs | 40 cycles | |
| 72°C extension | 30 secs | ||
| Final Extension | 72°C | 5 minutes | |
For low Copies PCR reactions can be extended to 45 Cycles.
Packing : The above PCR premix is available in convenient small packs as well as large packs for bulk users. The pre-mix is supplied with an additional vial of DNase Free water.Pre-mixes with various concs of MgCl2 are available as mentioned below. Generally the 2.5mM Pre-mix is considered as universal, however for further optimization for your parameters you can try Premixes with other Concs of MgCl2
| Cat. No. | PRODUCT | Pack Size | Pack Insert |
| 110121 | 2 X Hot Start PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 1.5 mM final Mg at 1 X Conc | 1ml (50 rxns 50µl) | 110121P |
| 110122 | 2 X Hot Start PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 1.5 mM final Mg at 1 X Conc | 1ml x 2 (100 rxns 50µl) | 110122P |
| 110123 | 2 X Hot Start PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 2.5 mM final Mg at 1 X Conc | 1ml (50 rxns 50µl) | 110123P |
| 110124 | 2 X Hot Start PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 2.5 mM final Mg at 1 X Conc | 1ml x 2 (100 rxns 50µl) | 110124P |
| 110125 | 2 X Hot Start PCR-Premix: 500 rxns of 50µl or 1000 rxns of 25µl 2.5 mM final Mg at 1 X Conc | 1ml x 10 (500 rxns 50µl) | 110125P |
| 110126 | 2 X Hot Start PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 3.5 mM final Mg at 1 X Conc | 1ml (50 rxns 50µl) | 110126P |
| 110127 | 2 X Hot Start PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 3.5 mM final Mg at 1 X Conc | 1ml x 2 (100 rxns 50µl) | 110127P |
| 110128 | 2 X Hot Start PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 4 mM final Mg at 1 X Conc | 1ml (50 rxns 50µl) | 110128P |
| 110129 | 2 X Hot Start PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 4 mM final Mg at 1 X Conc | 1ml x 2 (100 rxns 50µl) | 110129P |
| 110130 | 2 X Hot Start PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 5 mM final Mg at 1 X Conc | 1ml x 2 (100 rxns 50µl) | 110130P |
| 110131 | 2 X Hot Start PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl with special additives for specialized applications | 1ml x 2 (100 rxns 50µl) | 110131P |