M-MuLV Reverse transcriptase is purified from E. Coli strain harboring a plasmid that directs the synthesis of modified form of Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase. M-MuLV is a RNA/ DNA Directed DNA Polymerase. The enzyme can synthesize complementary DNA strand initiating from a primer using either RNA (cDNA Synthesis) or single stranded DNA as template. This enzyme had been genetically altered to remove associated RNase H activity. Removal of RNase H activity resulted in an increase of full-length cDNA products. MW of Reverse is 69 kDa.

SPECIFICATIONS : Enzyme is supplied at a Concentration of 200U/µl along with one vial of 5 X buffer, one vial of additional 25mM MgCl2 & one vial of DNase free water.

STORAGE BUFFER : 50mM TrisHCL (pH 8.3), 1mM EDTA, 100mM NaCl, 0.1mM DTT, 0.1% Triton X-100, 50% glycerol

SUPPLIED REACTION BUFFER : (5 X) 250mM Tris HCL(pH 8.3), 500mM KCL, 50mM DTT, 15mM MgCl2

STORAGE : Recommended storage is at -20 °C.

APPLICATIONS : Suitable for cDNA synthesis and dideoxy DNA sequencing.