T4 DNA ligase catalyzes the formation of a phosphodiester bonds between 5' phosphate and 3' hyroxyl termini in duplex DNA/RNA. This enzyme can join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrid.
Source : T4 DNA ligase is isolated from a recombinant E.coli strains.
Unit definition : One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of Lambda DNA 30 minute at 16°C at 5' termini concentration of 0.12µM.
STORAGE BUFFER : 50 mM KCL, 10mM Tris-HCL(pH 7.4), 0.1mM EDTA, 1mM DTT, 50% Glycerol. Store at -20°C
CONCENTRATION : 1 unit/µl
APPLICATION : Cloning of restriction fragments, joining linkers and adapters to blunt ended DNA, gene synthesis
STORAGE : Recommended storage is at -20°C
| Cat. No. | PRODUCT | Pack Size | Pack Insert |
| 110341 | T4 ligase (with reaction buffer) | 100 U | 110341P |
| 110342 | T4 ligase (with reaction buffer) | 200 U | 110342P |