diagnosticsgenome.com Home Products Molecular Regents PCR PreMix Universal 2.5 X PCR-PreMix2.5 x PCR PRE-MIX is an optimized, ready to use PCR mixture of Taq DNA Polymerase, PCR buffer, MgCl2 and dNTP's. This 2.5x PCR pre-mix contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates.
SPECIFICATIONS : Supplied as 2.5x in liquid form so as to ensure that you can add your primers & Template DNA volumes to desired levels.
APPLICATIONS : Suitable for routine PCR for high workloads. Start up customers, for high throughput laboratories.
STORAGE : Recommended storage is at 0-20°C.
SUGGESTED DILUTION PROTOCOL for PCR :
| Component | 50 µl reaction Volume | 25 µl reaction volume | ||
| Volume | Final Conc. | Volume | Final Conc. | |
| 2.5 X PCR Premix | 20 µl | 1 X | 20 µl | 1 X |
| Forward primer | Variable | 0.1-1 µM | Variable | 0.1-1 µM |
| Reverse Primer | Variable | 0.1-1 µM | Variable | 0.1-1 µM |
| Template DNA | Variable | 10pg-1 µg | Variable | 10pg-1 µg |
| Sterile Deionized water | Up to 50 µl | ---- | Up to25 µl | ---- |
| Suggested Cycling protocol : | |||
| Initial Denaturation: | 95°C | 5 minutes | |
| Cycling | 95°C | 20 secs | |
| Annealing | 30 secs | 40 cycles | |
| 72°C extension | 30 secs | ||
| Final Extension | 72°C | 5 minutes | |
Packing : The above PCR premix is available in convenient small packs as well as large packs for bulk users.
The pre-mix is supplied with an additional vial of MgCl2 and one vial of DNase Free water. Also Pre-mixes with various concentrations of MgCl2 are available as mentioned below. Generally the 2.5mM Pre-mix is considered as universal however for further optimization for your parameters you can try Pre-mixes with other concentrations of MgCl2
| Cat. No. | PRODUCT | Pack Size | Pack Insert |
| 110110 | 2.5 X PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 1.5 mM final Mg at 1 X Conc. | 1ml (50 rxns 50µl) | 110110P |
| 110111 | 2.5 X PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 1.5 mM final Mg at 1 X Conc. | 1ml x 2 (100 rxns 50µl) | 110111P |
| 110112 | 2.5 X PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 2.5 mM final Mg at 1 X Conc. | 1ml (50 rxns 50µl) | 110112P |
| 110113 | 2.5 X PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 2.5 mM final Mg at 1 X Conc. | 1ml x 2 (100 rxns 50µl) | 110113P |
| 110114 | 2.5 X PCR-Premix: 500 rxns of 50µl or 1000 rxns of 25µl 2.5 mM final Mg at 1 X Conc. | 1ml x 10 (500 rxns 50µl) | 110114P |
| 110115 | 2.5 X PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 3.5 mM final Mg at 1 X Conc. | 1ml (50 rxns 50µl) | 110115P |
| 110116 | 2.5 X PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 3.5 mM final Mg at 1 X Conc. | 1ml x 2 (100 rxns 50µl) | 110116P |
| 110117 | 2.5 X PCR-Premix: 50 rxns of 50µl or 100 rxns of 25µl 4 mM final Mg at 1 X Conc. | 1ml (50 rxns 50µl) | 110117P |
| 110118 | 2.5 X PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 4 mM final Mg at 1 X Conc. | 1ml x 2 (100 rxns 50µl) | 110118P |
| 110119 | 2.5 X PCR-Premix: 100 rxns of 50µl or 200 rxns of 25µl 5 mM final Mg at 1 X Conc. | 1ml x 2 (100 rxns 50µl) | 110119P |